Longnon-codingRNAHUMThypomethylationpromoteslymphangiogenesisandmetastasisviaactivatingFOXK1transcriptionintriple-negativebreastcancer.长链非编码RNAHUMT低甲基化通过激活三阴性乳腺癌的FOXK1转录促进淋巴管生成和转移作者：ZhengS,YangLetal.JHematolOncol.Mar5;13(1):17.
Abstract
BACKGROUND
riple-negativebreastcancer(TNBC)isthemostmalignantsubtypeofbreastcancerwithhighlyinvasiveabilityandmetastaticnaturetothelymphnodes.Longnon-codingRNAs(lncRNAs)havebeenwidelyexploredincancertumorigenesisandprogression.However,theirrolesinTNBClymphnodemetastasisremainsrarelystudied.
METHODSheexpressionoflncRNAhighlyupregulatedinmetastaticTNBC(HUMT)incelllinesandtissueswasdetectedbyquantitativereal-timePCR(qRT-PCR)andinsituhybridization(ISH).RNAimmunoprecipitation(RIP)andRNApulldownwereusedtoverifytheinteractionbetweenlncRNAandprotein.Chromatinimmunoprecipitation(CHIP)anddCas9-gRNA-guidedchromatinimmunoprecipitation(dCas9-CHIP)wereconductedtoidentifythespecificbindingsiteofHUMT-YBX1
